| Research
Scientist
Company creates proprietary,
cell-based assays
using fluorescence to define temporal and spatial activity of specific targets and cell
processes as
a key step in the drug discovery process. The
Research Scientist will be part of a team
identifying new molecular-genetic and molecular-biochemical reagents for proprietary
high content screening systems. The research
team will consist of cell biologists, biochemists,
and molecular biologists who will continuously
interact with the development team to provide advanced fluorescent reagents. The reagent
research team will develop fluorescent reagents
to dissect chemical and molecular pathways in
living cells. The team will develop fluorescent
reagents to measure the dynamics of metabolites, macromolecules, and organelles within
living cells. These reagents include molecular-genetic
fluorophores as well as molecular-biochemical,
small molecule indicators, and fluorescent protein biosensors. Requirements: Requires a
Ph.D.
and at least three years of cell biology and
biochemistry post-doctoral experience in either academia or industry. Specific skills
include
protein structure-function analysis, recombinant
and native protein expression, purification, characterization, and tissue culture and
molecular manipulation of primary and established insect and higher vertebrate cell lines.
Or alternatively, at least three years of cell biology
and biophysics post-doctoral experience in either academia or industry. Specific skills
include fluorescence spectroscopy (steady state,
polarization, and dynamic methods) of
small molecules and macromolecules as well
as the application of fluorescent reagents and
light microscopy to physiology research in both
fixed and live cell preparations.
Or alternatively, the preparation of molecule-genetic-based fluorophore
chimeras (e.g., GFP) and their expression in
prokaryotic and eukaryotic (yeast, insect, and mammalian) cells, preparation and
maintenance
of stable-transfected cell lines, and measurement
of the dynamics of fluorescence-based
reagents in living cells.
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